Following infection with any of the three bacteria, breeding activities should only be resumed with approval from the attending veterinary surgeon, and in the case of the CEMO, of the APHA, who must be satisfied that infected and in-contact horses have been investigated, treated as appropriate and subsequently cleared on the basis of negative swabs.
The first post treatment swabs should be taken seven or more days after the treatment has ended. All post treatment swabs should be tested by aerobic and microaerophilic culture and/or by PCR. All positive isolates of K. pneumoniae should be capsule typed where they are identified on post-treatment samples, irrespective of whether pathogenic K. pneumoniae was isolated prior to treatment. All Klebsiella pneumoniae PCR results will need culture tests also performed to provide bacteria for capsule typing.
Three clitoral swabs should be taken at intervals of at least seven days and three endometrial swabs should be taken during the next three oestrous periods. In respect of maiden mares, only on endometrial swab is needed. All results must be confirmed as negative before any breeding activities resume. If any result is positive, further investigation should be undertaken in conjunction with the attending veterinary surgeon.
Three sets of penile swabs should be taken at intervals of at least seven days and negative results confirmed. Thereafter, the first three mares mated or inseminated by the stallion should have clitoral swabs taken three times at intervals of at least seven days, starting two days after mating or insemination. If any of these swab results are positive, breeding activities should cease pending further investigation in conjunction with the attending veterinary surgeon.